Composite
ADH2-kivD-
Part:BBa_K2588025:Design
Designed by: Dominika Wawrzyniak, Daniel Wedemeyer Group: iGEM18_Hamburg (2018-10-05)
3-methyl-1-butanol synthesis module, arabinose-induced
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
Illegal BamHI site found at 7384
Illegal XhoI site found at 207 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 965
Illegal AgeI site found at 6122 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 7210
Illegal SapI.rc site found at 4042
Design Notes
non
Source
Composite of registry parts, genes amplified by PCR from E. coli genomic DNA, and synthetic DNA based on codon-optimized genes from Lactococcus lactis and Saccharomyces cerevisiae.